Health Sciences Sequencing Core
All PITT/UPMC prices below are effective May 1, 2024.
| Sample Preparation | Unit | Price |
| RNA/DNA extraction tissue / cell pellet | Sample | $37 |
| RNA/DNA extraction biofluid / FFPE / miRNA | Sample | $54 |
| Quality checks | Unit | Price |
| Agilent TapeStation DNA or RNA | Sample | $10 |
| Qubit Fluorometric Quantification | Sample | $5 |
| RNA Library preparation | Unit | Price |
| llumina Stranded Total Ligation (Ribo-Zero Plus) | Sample | $250 |
| llumina Stranded Total Ligation (Ribo-Zero Plus) ≥ 48 Samples | Sample | $200 |
| llumina Stranded mRNA (PolyA+) 1-47 | Sample | $165 |
| llumina Stranded mRNA (PolyA+) ≥ 48 Samples | Sample | $115 |
| SMARTer TCR Profiling (Human / mouse) | Sample | $165 |
| SMART-Seq Stranded Total RNA (Ultra Low Input) | Sample | $175 |
| SMART-Seq Stranded Total RNA (Ultra Low Input) ≥ 48 Samples | Sample | $130 |
| SMART-Seq HT Ultra Low Input | Sample | $115 |
| SMART-Seq HT Ultra Low Input ≥ 48 Samples | Sample | $90 |
| Lexogen 3' Quantseq | Sample | $165 |
| QIAseq small RNA | Sample | $250 |
| QIAseq small RNA ≥ 48 Samples | Sample | $200 |
| Nanostring GeoMX (per plate) | Plate | $200 |
| High-capacity library pooling | Sample | $17 |
| DNA Library Preparation | Unit | Price |
| Illumina DNA Prep/Nextera XT | Sample | $115 |
| Illumina DNA Prep/Nextera XT ≥ 48 Samples | Sample | $90 |
| Illumina DNA PCR-Free Prep (WGS) | Sample | $90 |
| ChIP-seq (Library prep only) | Sample | $175 |
| ATAC-Seq | Sample | $290 |
| Visium Spatial Transcriptomics | Unit | Price |
| Visium Fresh Frozen Tissue Permeabilization (2 tissues) | Run | $2,400 |
| Visium Fresh Frozen Spatial Gene Expression, 6.5mm capture (4 tissues) | Run | $7,900 |
| Visium CytAssist FFPE/Fresh, 6.5mm capture (4 tissues) | Run | $9,900 |
| Visium CytAssist FFPE/Fresh, 11mm capture (2 tissues) | Run | $10,000 |
| Visium CytAssist Protein Expression, 6.5mm capture (4 tissues) | Run | $3,600 |
| Visium HD CytAssist FFPE, 6.5mm capture (4 tissues) | Run | $16,400 |
| Curio Slide-seq Spatial Transcriptomics | Unit | Price |
| Curio Slide-seq 3x3 (4 tissues) | Run | $9,200 |
| Curio Slide-seq 10x10 (4 tissues) | Run | $22,200 |
| NextSeq 2000 Sequencing Runs | Unit | Price |
| P1 100 cycle kit (100M) | Run | $1,300 |
| P1 300 cycle kit (100M) | Run | $1,800 |
| P1 600 cycle kit (100M) | Run | $2,500 |
| P2 100 cycle kit (400M) | Run | $1,900 |
| P2 200 cycle kit (400M) | Run | $3,450 |
| P2 300 cycle kit (400M) | Run | $4,480 |
| P2 600 cycle kit (300M) | Run | $4,900 |
| P3 100 cycle kit (1200M) | Run | $4,000 |
| P3 200 cycle kit (1200M) | Run | $5,600 |
| P3 300 cycle kit (1200M) | Run | $7,350 |
| P4 50 cycle kit (1.8B) | Run | $3,100 |
| P4 100 cycle kit (1.8B) | Run | $3,600 |
| P4 200 cycle kit (1.8B) | Run | $4,960 |
| P4 300 cycle kit (1.8B) | Run | $6,170 |
| MiSeq Sequencing Runs | Unit | Price |
| MiSeq 150 cycle kit (25M) | Run | $1,470 |
| MiSeq 300 cycle kit (15M) | Run | $1,650 |
| MiSeq v3 600 cycle kit (25M) | Run | $2,300 |
| MiSeq v2 300 cycle kit MICRO (4M) | Run | $690 |
| Special Services | Unit | Price |
| Special Analysis/Services | Hour | $70 |
For external customers, please contact Health Sciences Sequencing Core directly
Single Cell Core
All prices below are effective January 1, 2024.
|
10X Genomics Single Cell Library Preparation |
Unit |
Price |
|
3’ or 5’ Single Cell RNA-Seq (Base-1st well) |
Sample |
$3,251 |
|
3’ or 5’ Single Cell RNA-Seq (2nd-8th well) |
Sample |
$2,090 |
|
Single Cell ATAC-Seq (Base-1st well) |
Sample |
$3,189 |
|
Single Cell ATAC-Seq (2nd-8th well) |
Sample |
$2,165 |
|
Single Cell Multiome (Base-1st well) |
Sample |
$3,626 |
|
Single Cell Multiome (2nd-8th well) |
Sample |
$3,319 |
|
Single Cell FLEX (Fixed RNA Single-Plex) per sample |
Reaction |
$2,352 |
|
Single Cell FLEX (Fixed RNA 4-Plex) per 4 samples |
Reaction |
$4,182 |
|
Single Cell FLEX (Fixed RNA 16-Plex) per 16 samples |
Reaction |
$10,809 |
|
Additional Library Preparation |
Unit |
Price |
|
BCR/TCR Library, mouse or human (add on to 5’ scRNAseq) |
Sample |
$321 |
|
Feature Barcoding (Cite-Seq or hashtagging for 3’ and 5’ scRNAseq) |
Sample |
$326 |
|
Library QC |
Unit |
Price |
|
Qubit Quantification |
Sample |
$7 |
|
TapeStation cDNA and Library QC |
Sample |
$32 |
|
Additional Services |
Unit |
Price |
|
Single Cell Data Analysis |
Hour |
$76 |
|
Single Cell Hourly Service (Late fee for processing after 5pm) |
Hour |
$63 |
Please contact heidi.monroe@pitt.edu for a project quote.