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Work Flows Across the Cores

 

The HSSC, Single Cell Core, and HTGC (formerly, the UPMC Genome Center) work together to provide sequencing in the most cost-effective manner. For large projects with many samples, projects may start in the HSSC and be sent to HTGC for sequencing for lower cost per sample on high throughput sequencing machines. The work flow diagram and table below aims to provide users with the most common project types and the cores that are optimally setup to handle each project type. For some projects this may depend on quality of input or sample number. If you are still unsure which core is right for your project, please contact us.

You may download the PDF version here too, or refer to the Chart below when needing services.

Service Need

Primary Core

Possible Secondary Core

Single Cell Assays

Single Cell Core

HSSC or HTGC

High Input High throughput RNAseq

HSSC or HTGC

 

Low input RNAseq

HSSC

HTGC

DNA for WES or WGS

HTGC

 

Pre-mixed libraries

HSSC or HTGC

 

Spatial Transcriptomics

HSSC

HTGC

Extractions (blood/tissue/saliva)

HSSC

 

Epigenetics: ATACseq, ChIPseq

HSSC

HTGC

Clinical Testing

HTGC